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BSA was entrapped into PLGA 50:50 microspheres and the in vitro release study was performed. Then 1μg of microencapsulated antigen was subcutaneously administered to Balb/c mice and the serum Ig G response was compared to that obtained after the subcutaneous administration of the same amount of only free antigen or of free antigen emulsified 1:1 with Freund's complete adjuvant (FCA). The specific...
BSA was entrapped in particles of different sizes (200, 500 and 1000nm) prepared from poly(d,l-lactic-co-glycolic) acid by a double emulsion method. The particles were given, either intranasally, orally or subcutaneously, to Balb/c mice and the serum IgG, IgG1 and IgG2a response elicited was compared to that obtained by the subcutaneous administration of either free antigen, free antigen emulsified...
Previous studies have shown that in various mouse models of Alzheimer's disease (AD), amyloid β-protein (Aβ) antibodies generated by Aβ peptide immunization resulted in the prevention of Aβ plaque formation in brains of young mice, decreased Aβ plaque burdens in older mice and improved cognition. The purpose of this study was to optimize Aβ immunization protocols for future trials in transgenic mouse...
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