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We have cloned a full-length cDNA for testicular 20β-HSD in yellow catfish. The validated 20β-HSD cDNA full-length sequence, 1141bp in length, contained a 108bp 5′-untranslated region (UTR), a 202bp 3′-UTR with an AATAAAA frame, and an 831bp open reading frame (ORF) which encoded a propeptide of 277 amino acid residues. The enzyme shows the highest structural homology with that of zebrafish, and rainbow...
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