We report a novel sensing platform for biosensor and pseudocapacitor application based on electron beam (EB) irradiated polypyrrole/cerium-nickel@modified guar gum (EB-PPy/Ce-Ni@MGG) nanobiocomposite. The EB irradiated PPy nanospheres (NSs) and Ce-Ni bimetallic NSs prepared are functionalized into the amine MGG matrix film for the improvisation of energy storage as well as charge transfer mechanism. MGG film also found to be an effective conducting material in composite formation like reduced graphene oxide (r-GO) with excellent electrocatalytic activity. The nanobiocomposite was confirmed by SEM, EDAX, XRD, Raman and FT-IR analysis. Interestingly, the EB-PPy/Ce-Ni@MGG modified glassy carbon electrode exhibited an excellent electrocatalytic activity towards the simultaneous sensing of hydroquinone, catechol, resorcinol and nitrite with lower detection limits as 87nM, 84nM, 518nM and 786nM. Furthermore, EB-PPy/Ce-Ni@MGG modified Ni foam electrode was evaluated in 1M NaOH by cyclic voltammetry and galvanostatic charge-discharge methods for pseudocapacitor application. The Ce-Ni bimetal NSs in the EB-PPy@MGG shows a significant improvisation of specific capacitance to 605Fg−1 at a current density of 1Ag−1. The optimized electrolyte concentration, current density, scan rate and cycle stability shows promising results to low cost energy storage with wearable power device fabrication.