The prospective study was carried out to document the change of antibody level to small ruminant lentivirus (SRLV) in chronically infected pregnant does. Thirteen dairy goats of Polish White Improved and Polish Fawn Improved breeds, asymptomatically infected with SRLV for at least a year, were enrolled. The goats were blood-sampled at mating, then four times during pregnancy, 2 weeks before kidding, at kidding and monthly for three months postpartum. Antibody titers to SRLV were determined by screening sera in increasing dilutions with three different commercial ELISAs: indirect whole-virus antigen (wELISA), indirect p28-transmembrane antigen (p28-TM-ELISA) and competitive gp135 (SU-ELISA). Then, the reciprocal of the greatest dilution at which a serum yielded the result greater than the cut-off of the test was considered the end-point antibody titer. Compared to the level at mating antibody titers significantly fell at kidding in all three tests. Significant decrease in antibody titer was observed for the longest time in SU-ELISA and for the shortest time in p28-TM-ELISA. At kidding false negative results were observed in two ELISAs (p28-TM-ELISA and SU-ELISA) and 3 of 13 goats became seronegative at kidding in at least one ELISA. At least four-fold fall in antibody titer between mating and kidding was observed in wELISA in 6 goats, in p28-TM-ELISA in 4 goats and in SU-ELISA in 5 goats. None of goats showed at least four-fold decrease in all three tests. Fall in antibody titer to SRLV in the periparturient period can interfere with the results of serological screening of pregnant goats.