Bali Botanical Garden is the ex-situ plant conservation which has been used for research activities, environment education/ services and recreation. However, this site becomes a magnet of regional development in the surrounding Bedugul Basin. However, this is an isolated region which has limited spatial utilization. Land-use/ land Cover change with Landsat time series (1997 and 2014) can be figured out how this influences land conservation and conversion around it. The research method used digital image processing, GIS and field measurement/observation. Land-use/ Land Cover information was derived by supervised multispectral classification. NDVI analysis was used to classify the vegetation density. Field measurement made on the sample locations were selected based on purposive sampling method. It is as the green belt and green barrier which not only protecting the ecosystem in the upper but also controlling land changes in downstream. The natural region in this upperstream has well protected (94% constant, 5% lost and 1% increase of the vegetation cover) but this under area has changed by 25.46% as built areas.