To determine the differences in stem-cell properties, in hepatic differentiation, and in the effects of hydrogen sulfide (H 2 S) on hepatic differentiation between human bone-marrow stem cells (hBMC) and stem cells from human exfoliated deciduous tooth pulp (SHED). CD117 + cells were magnetically separated and subjected to hepatic differentiation. CD117 + cell lineages were characterized for transcription factors indicative of stem cells by qRT-PCR. For the last 9days of the differentiation, the test cells were exposed to 0.1ng/mL H 2 S. Immunocytochemistry and flow-cytometry of albumin, alpha-fetoprotein and carbamoyl phosphate synthetase were carried out after differentiation. Genes expressed in SHED were also expressed in BMC. No difference in expression level of hepatic markers was shown by immunofluorescence. SHED showed more positive cells than hBMC (p<0.01). H 2 S increased the number of positive cells in both cultures (p<0.01). Both urea concentration and glycogen synthesis which indicate the maturation level increased significantly after H 2 S exposure (p<0.001 and p<0.05, respectively).SHED and BMC have similar properties. The level of hepatic differentiation in SHED compared with BMC was the same or higher. H 2 S increased the level of hepatic differentiation.