On the example of selected sulfonamides and N4-acteyl-analogues we demonstrate that antibiotic substances can be removed from artificial wastewater using anodic microbial biofilms. Particularly sulfamethoxazole and sulfadiazine are completely or partly removed, respectively, by biofilms within 7days of batch operation. Thereby, the removal process is shown not to affect the anodic microbial biofilm performance. As further shown for N4-acetyl-sulfamethoxazole and N4-acetyl-sulfadiazine the microbial bioelectrochemical removal of these compounds does not proceed via the retransformation to the sulfonamides and is thus not leading to the environmentally relevant pharmaceutical reactivation, as often observed for aerobic degradation processes within wastewater treatment plants.