While various techniques for measuring particle size distributions (PSD) of particulate matter (PM) exist, there is no a single agreed upon standard or reference method for PM with different characteristics. This study investigated differences in the PSD measurements by four PSD analyzers: LS13 320 multi-wave length laser diffraction particle size analyzer, LS230 laser diffraction particle size analyzer, LA-300 laser scattering particle size analyzer, and Coulter Counter Multisizer3 (CCM3). Simultaneously collected total suspended particulate (TSP) samples in a commercial egg production house were analyzed by the four analyzers for PSDs. In addition, four types of testing powders (limestone, starch, No.3 micro aluminum, and No.5 micro aluminum) were also analyzed by these four PSD analyzers. The results suggest when comparing measured mass median diameters (MMDs) and geometric standard deviations (GSD) of the PSDs, the laser diffraction method (LS13 320, LS230 and LA-300) provided larger MMDs and broader distributions (GSDs) than the electrical sensing zone method (CCM3) for all samples. When comparing mass fractions of PM 10 and PM 2.5 between the measured values and the lognormal fitting values derived from the measured MMDs and GSDs, lognormal fitting method produced reasonably accurate PM 10 mass fraction estimations (within 5%), but it failed to produce accurate PM 2.5 mass fraction estimations. The measured PM 2.5 mass fractions significantly differed from the lognormal fitting PM 2.5 fractions and the mean differences reached as high as 95%. It is strongly recommended that when reporting a PSD of certain PM samples, in addition to MMD and GSD, the mass fractions of PM 10 and PM 2.5 should also be reported.