Chlorella sp. is a microalga that could be utilized effectively in the simultaneous biogas upgrade and biogas fluid nutrient removal. Mixed light-emitting diode (LED) light wavelength treatments (red:blue=1:9, 3:7, 5:5, 7:3, and 9:1) were more appropriate for microalgal growth than monochrome LED light wavelength treatments (monochrome red, blue, and white). The most appropriate mixture ratios for biogas upgrade were red:blue=7:3 and red:blue=5:5. For biogas fluid nutrient removal, the most appropriate mixture ratio was red:blue=5:5. Therefore, the optimal mixture ratio of LED light wavelengths was red:blue=5:5. Under this treatment, components of upgraded biogas content (v/v) were 93.68±3.25% CH 4 , 1.57±0.42% CO 2 , 0.99±0.06% O 2 , and 3.80±0.34% H 2 O. The highest biogas fluid removal efficiencies of chemical oxygen demand, total nitrogen, and total phosphorus reduction were 78.91±2.75%, 73.05±2.04%, and 67.54±1.46%, respectively.