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We have devised a simple method to purify mitotically active Schwann cells (SC) from peripheral nerves of adult mice. Nerves were predegenerated in vitro for 7 days and after dissociation cells were plated on poly-l-lysine/laminin coated dishes in N2 serum-free culture medium supplemented with forskolin and heregulin-β1. Primary cultures were purified from contaminating fibroblasts by magnetic cell...
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