A series of composite catalysts Ni/CeO 2 –ZrO 2 were prepared via impregnation method with Ni as the active metal. A laboratory-scale fixed-bed reactor was employed to investigate the catalyst performance during hydrogen production by steam reforming bio-oil aqueous fraction. Effects of water-to-bio-oil ratio (W/B), reaction temperature, and the loaded weight of Ni and Ce on the hydrogen production performance of Ni/CeO 2 –ZrO 2 catalysts were examined. The obtained results were compared with commercial nickel-based catalysts (Z417). The best performance of Ni/CeO 2 –ZrO 2 catalyst was observed when the Ni and Ce loaded weight were 12% and 7.5% respectively. At W/B = 4.9, T = 800 °C, H 2 yield reaches the highest of 69.7% and H 2 content of 61.8% were obtained. Under the same condition, H 2 yield and H 2 content were higher than commercial nickel-based catalysts (Z417).