Desmodesmus sp. was used in anaerobically digested wastewater (ADW) for nutrients removal and the biodiesel production was measured and compared using fed-batch cultivation was investigated and compared with batch cultivation. The Desmodesmus sp. was able to remove 236.143, 268.238 and 6.427mg/L of TN, NH4-N and PO4-P respectively after 40d of fed-batch cultivation, while in batch cultivation the quantities of TN, NH4-N and PO4-P removed were 33.331, 37.227 and 1.323mg/L. Biomass production of Desmodesmus sp. was also enhanced in fed-batch cultivation, when ADW loading was carried out every 2days; the biomass concentration peaked at 1.039g/L, which was three times higher than that obtained in batch cultivation (0.385g/L). The highest lipid production (261.8mg/L) was also recorded in fed-batch cultivation as compared to batch cultivation (83.3mg/L). Fed-batch cultivation of Desmodesmus sp. could provide effective control of nutrients limitation and/or ammonia inhibition on microalgae cultivation.