The possibility of constructing a hypothesis on albumin enantioselectivity allowing consistent interpretation of retention and selectivity data for d,l-monocarboxylic acids, d,l-dicarboxylic acids and d,l-amino acids, measured by liquid chromatography and capillary zone electrophoresis, is demonstrated. An explanation of effects caused by high temperature, mildly alkaline aqueous solutions and methanol, consistent with the explanation of the retention and selectivity data, is also possible. The hypothesis considers the conformational variability of albumin.