The study of the porcine immune system is of utmost importance for the better protection of the pig population against infectious agents. Monoclonal antibodies (Mabs) specific in standard assays are needed to quantify immunoglobulins (Ig) in biological fluids. The purposes of the study were to produce Mabs against porcine immunoglobulins, and light Ig chains, and to evaluate these Mabs in ELISAs and single radial immunodiffusion technique (RID) for detection of total Ig concentrations and/or specific antibodies. Porcine IgG1, IgG2, IgM and sIgA were isolated from colostral whey and Mabs to these isotypes and light chains were produced. Specificity and cross-reactivity of the Mabs was determined in ELISAs, immunoblotting, immunodiffusion and immunoelectrophoresis. Several Mabs were conjugated to horseradish peroxidase (HRP) using periodate method. Some Mabs showed high precipitating activity, and successfully used for quantitation of Ig by RID. This technique was used for quantitation of IgG, IgM, sIgA in colostrum and milk of the sows for study of immunogenicity of the vaccines against transmissible gastroenteritis virus and rotavirus in swine. In summary, Mabs specific for the major isotypes of porcine Ig are valuable reagents for the differentiation of IgG, IgM, IgA antibody responses and for delineating humoral immune responses in swine to viral and bacterial infections.