In this work the unique PbS nanosphere chains were synthesized by using DNA template with a simple precipitation method under mild conditions. The average diameter of the synthesized PbS nanospheres is about 30nm as characterized by transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). Cubic galena structure was confirmed by selected area electron diffraction (SAED) and X-ray diffraction (XRD) characterizations. Photoluminescence (PL) spectrum of PbS nanosphere chains showed that there were two emission peaks at 324nm and 360nm, respectively. The formation mechanism of PbS nanosphere chains was also proposed.