Glutaraldehyde activated chitosan beads bearing β-cyclodextrin cross-linked by 1,6-hexamethylene diisocyanate was prepared, and characterized by IR, XRD and TG-DTA. Its adsorption property of hydroquinone was investigated by spectrophotometry. The adsorption capacity increases with increasing temperature and pH value. The adsorption process can be better simulated by Freundlich isotherm equation (R2>0.996) and the thermodynamic parameters such as free energy change, enthalpy change and entropy change were calculated. The adsorption of hydroquinone on chitosan beads bearing β-cyclodextrin cross-linked by 1,6-hexamethylene diisocyanate is a spontaneous, endothermic and random process.