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The binding of Ca2+ to troponin C (TnC) in the troponin complex is a critical step regulating the thin filament, the actin–myosin interaction and cardiac contraction. Phosphorylation of the troponin complex is a key regulatory mechanism to match cardiac contraction to demand. Here we demonstrate that phosphorylation of the troponin I (TnI) subunit is simultaneously increased at Ser-150 and Ser-23/24...
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