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Inhibition of human [ 125 I]calcitonin gene-related peptide-I ([ 125 I]hCGRP-I) binding by human adrenomedullin (hADM), its N-terminal truncated fragments, CGRP and amylin, and cyclic AMP accumulation were examined in the human neuroblastoma cell line SK-N-MC. Binding of [ 125 I]hCGRP-I (125 pM) was inhibited by hCGRP-I, hADM(1-52), hADM(13-52),...
Adrenomedullin (ADM) is a new endogenous hypotensive and vasorelaxing peptide that may play an important role in the regulation of cardiovascular function. Although ADM was originally isolated from pheochromocytoma, ADM-like immunoreactivity has also been widely detected in various tissues, including the cardiovascular system. Based upon the reports that ADM mRNA and ADM-like immunoreactivity are...
Adrenomedullin (ADM), a vasodilatatory peptide contained in adrenal medulla, was found to induce a dose-dependent increase in aldosterone (ALDO) and corticosterone (B) release by the in situ perfused rat adrenal gland, along with a rise in the flow rate of the perfusion medium. The minimal effective dose for ALDO response was three and two orders of magnitude less than those able to evoke B and medium...
Responses to proadrenomedullin NH 2 -terminal 20 peptide (hPAMP), a truncated analogue [hPAMP (12-20)], and adrenomedullin (hADM) were investigated in the mesenteric vascular bed of the cat. Under constant-flow conditions, injections of hPAMP, hPAMP (12-20), and hADM caused dose-related decreases in mesenteric perfusion pressure. hADM was 100-fold more potent than hPAMP, and 1000-fold more...
Responses to a newly synthesized human adrenomedullin (hADM) analog, hADM(16-31), were investigated in the rat and cat. Unlike the full-sequence peptide, which has potent hypotensive activity, hADM(16-31) had pressor activity in the rat but not in the cat. Injection of hADM(16-31) in doses of 10-300 nmol/kg IV induced dose-dependent increases in systemic arterial pressure in the rat, and the peptide...
Takahashi, K., M. Sone, F. Satoh, O. Murakami, K. Totsune, H. Tanji, N. Sato, H. Ito and T. Mouri. Presence of adrenomedullin-like immunoreactivity in the human cerebrospinal fluid. Peptides 18(3) 459–461, 1997.—The presence of adrenomedullin-like immunoreactivity in the cerebrospinal fluid was studied by radioimmunoassay in 13 subjects with various neurological diseases. The concentrations of adrenomedullin-like...
Lewis, L. K., M. W. Smith, S. O. Brennan, T. G. Yandle, A. M. Richards and M. G. Nicholls. Degradation of human adrenomedullin(1–52) by plasma membrane enzymes and identification of metabolites. Peptides 18 (5) 733–739, 1997.—Very little is known about degradation or metabolism of adrenomedullin. To this end, we incubated adrenomedullin with ovine adrenal, kidney and lung plasma membrane preparations...
NUSSDORFER, G. G., G. P. ROSSI AND G. MAZZOCCHI.Role of adrenomedullin and related peptides in the regulation of the hypothalamo–pituitary–adrenal axis.PEPTIDES 18(7) 1079–1089, 1997.—Adrenomedullin (ADM) is a hypotensive peptide, originally isolated from human pheochromocytomas, and then found to be widely distributed in the various body systems. ADM derives from preproadrenomedullin, a 185-amino...
TAKAHASHI, K., F. SATOH, M. SONE, O. MURAKAMI, H. SASANO, T. MOURI AND S. SHIBAHARA.Expression of adrenomedullin mRNA in the human brain and pituitary.PEPTIDES 18(7) 1051–1053, 1997.—We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary,...
Takahashi, K., F. Satoh, E. Hara, M. Sone, O. Murakami, T. Kayama, T. Yoshimoto and S. Shibahara.Production and secretion of adrenomedullin from glial cell tumors and its effects on cAMP production.Peptides 18(8)1117-1124, 1997.-The expression of adrenomedullin (ADM) and its mRNA was studied in human glial cell tumors and cultured glioblastoma cell lines, T98G and A172. Northern blot analysis showed...
Moody, T. W., M. J. Miller, A. MARtinez, E. Unsworth and F. Cuttitta.Adrenomedullin binds with high affinity, elevates cyclic AMP, and stimulates c-fos mRNA in C6 glioma cells. Peptides 18(8) 1111-1115, 1997.-The effects of adrenomedullin (ADM) on C6 glioma cells were investigated. [ 125 I]ADM bound with high affinity (K d = 24 nM) to a single class of sites (B m ...
Sone, M., K. Takahashi, F. Satoh, O. Murakami, K. Totsune, M. Ohneda, H. Sasano, H. Ito and T. Mouri.Specific adrenomedullin binding sites in the human brain. Peptides 18(8) 1125-1129, 1997.-Binding sites for adrenomedullin in human brain were investigated and characterized by radioligand binding. Specific binding sites for adrenomedullin were present in every region of human brain (cerebral cortex,...
Disa, J., K. Dang, K. B. Tan and N. Aiyar. Interaction of adrenomedullin with calcitonin receptor in cultured human breast cancer cells, T 47D. Peptides 19(2) 247-251, 1998.-Human adrenomedullin (hADM), human calcitonin gene-related peptide (hCGRP), and salmon calcitonin (sCT)-activated adenylyl cyclase with EC 50 values of 132, 764, and 0.5 nM, respectively, in human breast cancer cell...
Immunoreactive-adrenomedullin concentrations and the expression of adrenomedullin mRNA were studied in the tumor tissues of adrenocortical tumors. Northern blot analysis showed the expression of adrenomedullin mRNA in tumor tissues of adrenocortical tumors, including aldosterone-producing adenomas, cortisol-producing adenomas, a non-functioning adenoma and adrenocortical carcinomas, as well as normal...
We have investigated the effect of adrenomedullin (AM) on restitution of mucosal integrity following damage in rat and human gastric mucosa, measuring the potential difference (PD) on a mucosal strip mounted on an Ussing chamber. Mucosal damage was induced by 0.5, 1.0, and 2.0 M NaCl solution, and it caused an immediate and significant decrease in PD. In the rat AM group, PD recovered significantly...
Adrenomedullin-like immunoreactivity in the hypothalamo-neurohypophysial tract in colchicine-treated and hypophysectomized rats was examined by immunohistochemistry. Adrenomedullin-like immunoreactive (AM-LI) neurons were localized in the hypothalamic areas, including the paraventricular nuclei and the supraoptic nuclei. Abundant AM-LI fibers and varicosities were found in the hypothalamo-neurohypophysial...
To explore the role of adrenomedullin (ADM) in pathophysiology of ischemic heart disease, we investigated the effects of hypoxia on the production and secretion of ADM in cultured human coronary artery endothelial cells. Treatment with hypoxia (5% CO 2 /94% N 2 /1% O 2 ) for 6 and 12 h increased expression levels of ADM mRNA 2.2-fold and fivefold compared with the normoxia...
To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 +/- 0.12 fmol/10 5 cells/24 h (n = 10). Reverse-phase...
Adrenomedullin (ADM) is a hypotensive peptide, highly expressed in the mammalian adrenal medulla, which belongs to a peptide superfamily including calcitonin gene-related peptide (CGRP) and amylin. Quantitative autoradiography demonstrated the presence of abundant [ 125 I]ADM binding sites in both zona glomerulosa (ZG) and adrenal medulla. ADM binding was selectively displaced by...
The study of two populations with a recent onset of type 2 diabetes showed that a subset of the patients had higher levels of adrenomedullin (AM) than the rest of the diabetics. In this subset, physiological elevations of AM might have triggered the disease in predisposed individuals. Diabetics showed higher levels of AM than healthy controls. In addition, glycemia was measured in diabetic rats after...
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