As nitric oxide (NO) is postulated to be a mediator of the effects of pre-slaughter stress on meat quality the aims of this experiment were to investigate the effects of modulating NO pharmacologically on meat quality of sedentary lambs. As pharmacological NO donors are prohibitively expensive to use in the lamb model l-Arginine, the substrate for NO synthase (NOS) was infused into lambs and increased NO production by ~30%. In a 2×2 factorial design we infused either l-Arginine (500mg/kg) or the NOS inhibitor L-N G nitroarginine methyl ester hydrochloride (L-NAME, 30mg/kg) 190min pre-slaughter and investigated meat quality in the Longissimus thoracis lumborum (LTL) or Semimembranosus (SM). The principal outcome of the experiment was that L-NAME inhibited proteolysis and reduced tenderness in the SM. These data indicate that events pre-slaughter that affect NO synthesis can influence meat tenderness, potentially via altered muscle metabolism or modulation of proteolytic enzymes.