The relationship between length of leisure time and periodontal condition is unknown. The aim of this cross-sectional study was to clarify the association between leisure time and periodontal states.This study was conducted on a cross-sectional sample of male (n=68) and female (n=30) Japanese healthy workers aged between 22 and 75 years. Periodontal states, including probing pocket depth, attachment level, bleeding on probing and serum antibody level for periodontal bacteria, and self-reported work conditions were assessed.Subjects with long leisure times on a weekday showed increased probing pocket depth and attachment loss compared to subjects with shorter free times. The serum antibody level of a major periodontal pathogen, Porphyromonas gingivalis, was higher in the long leisure group than in the short time group.Subjects with long leisure time showed aggregated periodontal condition consistent with increased serum antibody against periodontal bacteria compared to subjects with short free time.