Aim: The purpose of this study was to determine whether a combination of fibrin glue, β-tricalcium phosphate as a biodegradable (β-TCP) and mesenchymal stem cells would provide three-dimensional templates for bone growth resulting in new bone formation at heterotopic sites in the rat with plasticity.Material and Methods: Growing stem cells and developing matrices, explanted from the rat femur, were fragmented and mixed with fibrin glue in a syringe. The cells/β-TCP fibrin glue admixtures were injected into the subcutaneous space on the dorsum of the rat.Results: Eight weeks after implantation, gross morphology revealed a pearly opalescence and firm consistency. Histological inspections showed newly formed bone structures in all admixtures, but none in the control groups when only fibrin glue and β-TCP were injected. Osteopontin, a protein important in bone development, was identified by using antibodies in all cells/β-TCP fibrin glue admixtures.Conclusion: Mesenchymal stem cells/β-TCP fibrin glue admixtures can result in successful bone formation. This technique holds the promise of a minimally invasive means of generating autogenous bone to correct or reconstruct bony defects.