The feasibility of hydrogen production from mixed corn starch wastewaters was investigated to recover energy from the treatment of corn starch wastewater. Effects on hydrogen production using peptone, NH4Cl and a mixture of CSL and CGW as nitrogen sources were evaluated with supplement of GW. Experimental results showed that the optimum initial C/N ratio and total sugar concentration was 9.1 and 10.0 g/L respectively with the maximum hydrogen yield of 1.88 mol H2/mol glucose consumed by mixed culture of Bacillus sp. A1 and Brevumdimonas sp. B1. A modified Gompertz equation is able to describe the batch production of hydrogen from wastewater. The maximum hydrogen production rate (Rm) of 165.89 mL/L/h calculated by modified Gompertz equation was obtained using CSL/CGW as nitrogen source, which was 20.8 times higher than that using peptone as nitrogen source. The results revealed an excellent potential for hydrogen production using a mixture of CSL/CGW as a nitrogen source.