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To evaluate the contribution of multiple scattering in momentum-resolved electron energy-loss spectroscopy, the angular dependence of the intensities of multiple scattering was investigated and compared with elastic and inelastic singlescattering rofiles. From the experimental data the intensity ratio between multiple scattering and elastic scattering is derived. It can be used to estimate the contribution...
Colloidal gold is the most widely used electron dense marker in biological electron microscopy. The development of procedures for making gold particles of very defined sizes has made double or even multiple labelling possible using gold of two or more different sizes. Lately a new type of electron dense marker has been developed consisting of ligand-stabilized metal atom clusters rather than colloidal...
Crystalline bacterial cell surface layers (S-layers) are the outermost cell envelope component of many eubacteria and archaeobacteria. S-layers are composed of a single protein or glycoprotein species and exhibit oblique, square or hexagonal lattice symmetry. Pores passing through these monomolecular arrays show identical size and morphology, and functional groups are aligned in well-defined positions...
Sequential removal of the 33-, 23-, and 16-kDa extrinsic subunits of higher plant photosystem II (PSII) by various salt-washing procedures has been monitored by gel electrophoresis and oxygen evolution activity measurements. Structural changes induced by the subunit removal were followed by electron microscopy in conjunction with image processing of two-dimensional (2-D) crystals of PSII. In addition...
Energy filtering TEM (EFTEM) studies of a fibre-reinforced Ti-6A1-4V alloy designed for use in high temperature aerospace applications have been performed using a Philips CM20 TEM equipped with a Gatan imaging filter. The fibre employs a developmental duplex coating based upon a compliant layer of gadolinium metal and a gadolinium boride reaction layer both deposited by physical vapour deposition...
MDCK cells have been treated with a mixed surfactant at low concentrations to study the induced morphological changes. The most significant change at the light microscope level was the appearance of multiple large vesicles, which increased in size with time, up to ≈40 um in diameter. Vesicle formation was shown to be linked with the uptake of the fluid medium, as judged by the presence of FITC-dextran...
The feasibility and limitations of a 1024 × 1024 slow-scan charge-coupled device (CCD) camera were evaluated for imaging in a 400kV electron cryomicroscope. Catalase crystals and amorphous carbon film were used as test specimens. Using catalase crystals, it was found that the finite (24 μm) pixel size of the slow-scan CCD camera governs the ultimate resolution in the acquired images. For instance,...
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