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Oligonucleotide primers were designed for recovery of Ig H, κ and λ transcripts from porcine splenic cDNA. The products were cloned and scFvs constructed in a phage display vector. E. coli HB2151 was transformed with the constructs and upon induction, scFvs of the predicted molecular weight could be detected in culture supernatants by Western blotting and immunoprecipitation with anti-pig IgG. Bacteriophage...
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