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Sprague–Dawley rats 10–12 weeks of age were entrained to a standard light–dark cycle with lights turned on at 6am and off at 6pm. Variations of 24-h aqueous humor protein concentration were determined. Samples were taken every 4h (N=10–14) under the standard light–dark condition at 8pm, midnight, 4am, 8am, noon, and 4pm. Under an acute constant dark condition, when lights were not turned on at 6am,...
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