The purpose of this study was to investigate the effect of various bicycle seat designs on seat pressure and perceived stability in male and female cyclists using a unique field-based methodology. Thirty participants, comprising male and female cyclists, pedaled a bicycle at 118W over a 350m flat course under three different seat conditions: standard seat, a seat with a partial anterior cutout, and a seat with a complete anterior cutout. The pressure between the bicycle seat and perineum of the cyclist was collected with a remote pressure-sensing mat, and perceived stability was assessed using a continuous visual analogue scale. Anterior seat pressure and stability values for the complete cutout seat were significantly lower (p<0.05; 62–101%) than values for the standard and partial cutout designs. These findings were consistent between males and females. Our results would support the contention that the choice of saddle design should not be dictated by interface pressure alone since optimal anterior seat pressure and perceived seat stability appear to be inversely related.