To evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on the haematological dynamics of an acute inflammatory process after an iatrogenic muscular lesion.Controlled laboratory study.Research laboratory.Eighteen male Wistar rats (weight 350 to 450g) were submitted to surgical incision in the biceps femoris muscle (approximately 50%), and subsequently subdivided into control (n=9) and LIPUS (n=9) groups.Ultrasound (1.0MHz) was applied at the lesion site in the pulsed mode (2ms on, 8ms off) at 0.4W/cm 2 for 3minutes at 1, 8 and 24hours post-surgery.In these periods, blood was collected through venepuncture of the retro-orbital plexus and evaluated for different types of leukocytes and erythrocytes.LIPUS reduced the total leukocyte count at 1, 8 and 24hours post-surgery (1hour, control 9017.2±481×10 3 /mm 3 vs LIPUS 6189.8±450×10 3 /mm 3 ; 8hours, control 8078.2±501×10 3 /mm 3 vs LIPUS 5371.3±378×10 3 /mm 3 ; 24hours, control 8192.3±646×10 3 /mm 3 vs LIPUS 6059.1±503×10 3 /mm 3 ; P<0.001). The monocyte count was reduced at 8 and 24hours post-surgery (8hours, control 815.5±126×10 3 /mm 3 vs LIPUS 375.4±70×10 3 /mm 3 ; 24hours, control 875.3±124×10 3 /mm 3 vs LIPUS 564.7±56×10 3 /mm 3 ; P<0.001). The number of segmented neutrophils was only reduced at 1hour post-surgery (control 5033.1±397×10 3 /mm 3 vs LIPUS 3594.8±191×10 3 /mm 3 ; P=0.006), and the lymphocyte count was only reduced at 8hours post-surgery (control 4759.7±459×10 3 /mm 3 vs LIPUS 2584.1±356×10 3 /mm 3 ; P=0.003). Changes were not observed in the concentrations of young neutrophils, polibocytes and erythrocytes.LIPUS reduced aspects of the inflammatory process following an acute incisional muscular lesion.