A near-infrared diode laser spectrometer was used in the laboratory to measure accurately the molecular parameters of the water vapor isotopologues, H 2 16 O, H 2 17 O, H 2 18 O and HDO, in the 2.64μm region. The work was made with a new-generation distributed-feedback antimonide-based multi-quantum wells diode laser. The selected spectral range as well as the used laser technology is particularly interesting for the in situ monitoring of the water vapor isotopologues in the lower Martian atmosphere. In the laboratory, it was possible to carefully investigate with one single laser, four lines of H 2 16 O, two lines of H 2 18 O, one line of H 2 17 O and one line of HDO. The corresponding line strengths measurements are reported and thoroughly compared to existing databases. Finally, we discuss the design of a version of the TDLAS laser diode sensor, dedicated to the in situ monitoring of the H 2 O isotopologues on Mars.