A frequent feature of microtubule organisation in living systems is that it can be triggered by a variety of biochemical or physical factors. Under appropriate conditions, in vitro microtubule preparations self-organise by a reaction–diffusion process in which self-organisation depends upon, and can be triggered by, weak external physical factors such as gravity. Here, we show that self-organisation is also strongly dependent upon the presence of a high magnetic field, for a brief critical period early in the process, and before any self-organised pattern is visible. These results provide evidence that external physical factors trigger self-organisation by way of an orientational bias that breaks the symmetry of the reaction–diffusion process. As microtubule organisation is central to many cell functions, this behaviour provides a mechanism by which strong magnetic fields can intervene in biological processes.