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To investigate the phenotypic evolution of West Nile virus (WNV) in California, we competed sixteen isolates made during 2007–08 against COAV997-5nt, a genetically marked clone from the founding 2003 California isolate COAV997-2003. Using in vivo fitness competitions in House Finches (HOFI) and Culex tarsalis mosquitoes, we found that the majority of WNV WN02 and SW03 genotype isolates exhibited elevated...
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