Cyclohexene epoxidation with H 2 O 2 was performed over RuO 2 /TiO 2 under various conditions. The support TiO 2 prepared by surfactant-assisted templating sol–gel method is nanocrystalline and has mesoporous structure with narrow monomodal pore size distribution. An activator of 1mol% RuO 2 was loaded onto the synthesized TiO 2 by incipient wetness impregnation method. The resultant catalyst was characterized by XRD, N 2 adsorption–desorption and TEM analyses. Reaction conditions in the epoxidation reaction, namely reaction temperature, amount of catalyst and concentration of H 2 O 2 in terms of cyclohexene/H 2 O 2 ratio, were systematically optimized to obtain maximum selectivity to cyclohexene oxide.