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We examined the ability of rIL-13 to regulate rIL-lα induced IL-1β, IL-1 receptor antagonist (IL-1ra) and IL-8 production in cultured peripheral blood mononuclear cells (PBMC), endothelial cells and fibroblasts. Furthermore, we examined whether rIL-13 could influence the production of the arachidonic acid products LTB4, 12-HETE and 15-HETE by PBMC. rIL-1α-stimulated PBMC cultures secreted high levels...
Although interleukin (IL)-10 inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) and blocked stimulation of alloreactive T cell clones (TCC) by peripheral blood mononuclear cells (PBMC), the cells surviving culture with IL-10 showed enhanced viability. A minority of IL-2-dependent T cell lines, moreover, incorporated tritiated thymidine when cultured with IL-10 alone; their proliferation...
Weight loss and impaired nutritional status are associated with increased complications following surgery. This study aimed to assess the effect of nutritional status on the magnitude of the acute phase protein response, and determine if this is associated with changes in the magnitude of the related cytokine responses. Nineteen patients (10 wellnourished, 9 malnourished on the basis of body composition)...
We report the existence of a protein in normal human urine that binds, specifically and with high affinity (Kd∼ 1.9 nM) to human interleukin 4 (IL-4) and thereby prevents the binding of the cytokine to the high affinity receptor. The urinary binding protein has a molecular weight of 40 kD and 23 kD after deglycosylation. The binding protein has been purified by affinity chromatography. It inhibited...
We have recently shown that interleukin 1β (IL-1β) directly reduces the number of adipocytes in cultures of human bone marrow (BM) stromal cells. The aim of the present study was to establish the mechanisms involved in this IL-1 effect and thereby assess its importance in BM pathophysiology. Direct morphological observation showed that indi-vidual adipocytes lost their fat in the presence of IL-1β...
Cytokine gene expression was investigated in the germinal centreconstituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of non-germinal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1α, IL-1β, IL-2, IL-4,...
Chronic inflammation and degradation of connective tissue in the course of periodontitis are maintained by bacterial products such as lipopolysaccharides (LPS), which probably act via inflammation mediators, e.g. cytokines. We investigated the effects of lipopolysaccharide (LPS) fromE. coliand mouse recombinant interleukin 1α (mrIL-1) on chondrogenesis, endochondral mineralization, matrix metalloproteinase...
In human interleukin 4(IL-4), the carboxyl and amino termini of the 129 amino acid hormone are close to each other and this region is believed to be important for binding to the IL-4 receptor (IL-4r). We constructed plasmids encoding circularly permuted IL-4 mutants with the peptide Gly-Gly-Asn-Gly-Gly (GGNGG) joining the carboxyl to the amino terminus and with new amino and carboxyl termini elsewhere...
We have investigated the binding of interleukin 7 (IL-7) to sulfated glycosaminoglycans and evaluated its biological consequences. IL-7 binds to heparin and heparan sulfate, to a lesser extent to dermatan sulfate and does not bind to chondroitin sulfate. It was eluted from heparin by 0.3–0.6 M NaCl and from heparan sulfate by <0.3M NaCl. We also measured the affinity of IL-7 for heparin using an...
During the process of placental implantation, sessile villous trophoblast cells migrate from the villi into the decidua as isolated motile extravillous trophoblast cells. There is differential expression of the epidermal growth factor-receptor (EGF-R) and c-erbB2 proteins on villous and extravillous trophoblast populations. Using monoclonal antibodies to EGF-R and c-erbB2, we have obtained highly...
Nonsteroidal anti-inflammatory drugs (NSAIDs) are well known as immunoregulators, but the mechanisms of their action are not fully explained by the inhibition of PG synthesis. We have investigated the effect of NSAIDs on cytokine production in human PBMC and T cell clones (TCC). NSAIDs up-regulated TNF, IFN-γ and IL-2 production at both the mRNA and protein levels, and IL-12 expression at the mRNA...
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