Antianemic medicament Ascofer® and ferrous gluconate, its basic iron bearing ingredient, were studied with the use of Mössbauer spectroscopy. Room temperature spectra gave a clear evidence that two phases of iron were present viz. ferrous (Fe 2+ ) as a major one with a contribution of ∼85±5%, and ferric (Fe 3+ ) whose contribution was found to be ∼15±5%. However, the actual values of the contributions of the two kinds of the iron ions in Ascofer® were shown to depend on sample’s age: the abundance of Fe 2+ ions increased with time by ∼10% after 51months, while that of Fe 3+ decreased by the same amount. This means that an internal reduction of Fe 3+ ions took place. The ferrous ions were shown to occupy three different sites with populations of ∼75–79% for site 1, and ∼8–23% for site 2, and ∼1–13% for site 3.