The isotopically labelled S-3-(hexan-1-ol)-glutathione d 2 /d 3 (G3MHd 2 /d 3 ) was synthesized with a strategy based on acid-labile protecting groups. The natural analogue of this compound could be a precursor of 3-mercaptohexanol, a varietal thiol reminiscent of grape fruit, released during alcoholic fermentation. In a first time, deuterated glutathione conjugate was used to perform identification and quantification by stable isotope dilution assay of G3MH in musts from several varieties: Sauvignon, Riesling and Gewurztraminer.In a second time, synthetic and natural musts (Sauvignon Blanc) were spiked by G3MHd 2 /d 3 at different levels from 12 to 150μgL −1 to demonstrate the relationship between 3-mercaptohexan-1-ol (3MH) and G3MH. GC–ITMS/MS analysis of the resulting wines clearly showed the formation of the corresponding deuterated 3MH, that proves the direct connection with G3MH under enological conditions. In Sauvignon Blanc grape juice, a conversion yield of 4.4% was found in duplicate for an initial spiking of G3MH d 2 /d 3 equivalent to 12μgL −1 .