The object of this study was to determine if canal length is altered as a result of straight-line access (SLA) and coronal flaring (CF). Selected were 86 canals of extracted molars and premolars from two groups: straight or severely curved (Schneider curvature <5 degrees and >20 degrees). The reference cusp tip and root-end were flattened to produce reproducible measurements. A #10 file was placed such that the tip extended slightly beyond the apex, with the handle on the referenced cusp. The amount of file protrusion was measured with a stereomicroscope. Then, SLA and CF were performed and the corresponding file replaced to the same coronal reference position. Apical file protrusion was measured again. The change in canal length was determined by the difference in the pre- and post-SLA/CF measurements. A Wilcoxon signed rank test statistically verified that there was a measurable, significant (p < 0.001) change in canal length after SLA and CF. The mean change overall was slight, with a decrease of 0.17 mm. Severe curvature had a slightly greater, significant effect on the amount of change. Tooth type had no significant effect. Changes in working length from SLA and CF, although statistically significant, were very small and clinically unimportant.