The effect of thermal, ultraviolet-c, and high pressure treatments on colour, browning degree, dynamic viscosity, and lycopene content of watermelon juice was evaluated based on its pectin methylesterase residual level. Each treatment had a different impact on parameters studied. Ultraviolet-c treatments were rapid and effective to inactivate the pectin methylesterase of the watermelon juice compared to the thermal and high pressure treatments in the same time and temperature. High pressure treatments at 600MPa kept the colour of the treated watermelon juice close to an untreated one, and that at 600–900MPa held the browning degree and dynamic viscosity of the treated watermelon juice comparable to an untreated one. Moreover, the high pressure treatment had a slight impact on the all-trans-lycopene, total cis-lycopene, and total lycopene concentration of the watermelon juice compared to the other treatments. In summary, the high pressure treatment showed the lowest changes in colour, dynamic viscosity, browning degree, and lycopene content of the treated watermelon juice amongst the three treatments.