The low productivity of microalgae has restricted scale-up application of microalgae-based biodiesel processes. Diethyl aminoethyl hexanoate (DA-6) was investigated to enhance the biomass and metabolite productivity. At a very low concentration (10−7M) DA-6 made Chlorella ellipsoidea SDEC-11 and Scenedesmus quadricauda SDEC-13 obtain enlarged cell size, 114mgL−1d−1, 101mgL−1d−1 biomass productivity and 39.13mgL−1d−1, 32.69mgL−1d−1 lipid productivity, respectively. Biomass and lipid productivity of SDEC-11 and SDEC-13 were 100mgL−1d−1 and 30.05mgL−1d−1, 94mgL−1d−1 and 28.43mgL−1d−1, respectively, without DA-6. Twice hormone dose in 10−6M DA-6 medium resulted in higher biomass productivity (106mgL−1d−1) and longer exponential growth of SDEC-13. DA-6 also ensured the property of microalgae biodiesel to meet the EN 14214 standard. The current investigation demonstrated that DA-6 accelerated the microalgae growth and simultaneously improved the quality and quantity of lipid for biodiesel production.