Objective: To develop the representative fingerprint for the quality control of placenta polypeptide injection.The chromatographic separation was performed using a Phenomenex Gemini C18 column (250mm×4.6mm, 5μm) maintained at 30°C. 0.1% aqueous trifluoroacetic acid (Solvent A) and acetonitrile contained 0.1% TFA (Solvent B) were used as mobile phase with a gradient elution. Detection wavelength was 280nm with the sample injection volume of 50μL; the flow rate was 1.0mL/min. The fingerprints of different samples were investigated by similarity analysis.Nine peaks were identified as the characteristic common peaks. The similarities of the fingerprints of the 10 batches of samples were above 0.992.This method showed high precision and good repeatability, and provided the basis for the improvement of the quality control of placenta polypeptide injection.