An important breeding target for persimmon is to achieve elite cultivars of the pollination constant non-astringent (PCNA) type. The RO2 SCAR marker has been established to identify the trait of Chinese PCNA type by Ikegami et al. [J. Jpn. Soc. Hort. Sci. 80 (2011) 150–155]. Here, the informativeness of RO2 was tested in all Chinese PCNA types and two F 1 populations bred from crosses between a non-PCNA maternal parent and either a Chinese PCNA type or an androecious male parent. The RO2 marker was detected in ten Chinese PCNA types and six androecious genotypes, but not in any of the non-PNCA and Japanese PCNA types. The segregation behavior of RO2 in both F 1 populations was consistent with a 1:1 ratio as previously reported by Ikegami et al. The presence of the RO2 marker among androecious material indicates that they also have potential as donor of non-astringency trait of Chinese PCNA type.