Lucigenin (bis-N-methylacridinium nitrate) is an acridine-based probe that has been extensively used to detect reactive oxygen species (ROs) and has been shown to generate chemiluminescence in many biological materials. N-methylacridone is known to be the main emitter of the lucigenin's chemiluminescent system. This work investigates the distribution of synthesized 10-alkyl-9(10H)-acridone (-methyl, -butyl and -decyl) probes and quenchers (Cu 2 + , MV 2 + , BeV 2 + , Propy + and DoPy + ) in anionic micellar solution of sodium dodecyl sulfate (SDS).The binding ability of 10-alkyl-9(10H)-acridone to a serum lipoprotein (BSA)was investigated.