Nitrous oxide (N 2 O) emissions from nitritation reactors receiving real anaerobic sludge digestion liquor have been reported to be substantially higher than those from reactors receiving synthetic digestion liquor. This study aims to identify the causes for the difference, and to develop strategies to reduce N 2 O emissions from reactors treating real digestion liquor. Two sequencing batch reactors (SBRs) performing nitritation, fed with real (SBR-R) and synthetic (SBR-S) digestion liquors, respectively, were employed. The N 2 O emission factors for SBR-R and SBR-S were determined to be 3.12% and 0.80% of the NH4+-N oxidized, respectively. Heterotrophic denitrification supported by the organic carbon present in the real digestion liquor was found to be the key contributor to the higher N 2 O emission from SBR-R. Heterotrophic nitrite reduction likely stopped at N 2 O (rather than N 2 ), with a hypothesised cause being free nitrous acid inhibition. This implies that all nitrite reduced by heterotrophic bacteria was converted to and emitted as N 2 O. Increasing dissolved oxygen (DO) concentration from 0.5 to 1.0 mg/L, or above, decreased aerobic N 2 O production from 2.0% to 0.5% in SBR-R, whereas aerobic N 2 O production in SBR-S remained almost unchanged (at approximately 0.5%). We hypothesised that DO at 1 mg/L or above suppressed heterotrophic nitrite reduction thus reduced aerobic heterotrophic N 2 O production. We recommend that DO in a nitritation system receiving anaerobic sludge digestion liquor should be maintained at approximately 1 mg/L to minimise N 2 O emission.