The brain-gut-axis has been studied as a modulating factor of immune and behavioral responses throughout the course of infectious diseases. To analyze these relationships, 25 broilers were divided into 5 groups: naive group (N), thioglycolate group (T), thioglycolate heat stressed group (T/HS35), infected group (I) and infected heat stressed group (I/HS35). The broilers of I and I/HS35 groups were infected with Clostridium perfringens from the ±15th to the 19th day of life. Heat Stress (35±1°C) was constantly applied to the stressed groups from the ±14th to the 19th day of life. Heat stress induced an increase in the behavioral scores of the chickens of the T/HS35 and I/HS35 groups in comparison to animals of the N and T-groups (p<0.05). Results showed that heat stress and C. perfringens infection (I/HS35 group) also induced a significant increase in c-fos reactive neurons (c-fos-ir) in the paraventricular nucleus of the hypothalamus, and a decreased c-fos-ir in the amygdala and globus pallidus in the animals. Statistical differences weren’t found in c-fos-ir in the preoptic medial nucleus between infected groups. However, these differences were accompanied by a decreased score of intestinal necrosis in the chickens of the I/HS35 group in relation to broilers of the I group (p<0.05). These data display a brain-gut axis involvement in the modulation of behavioral responses to heat stress and intestinal infection in broilers.