Beetroots are good source of functional betalain pigment inherent with antioxidant activity and TPC. The feasibility of spray drying was devised along with the effects of whey protein concentrate (WPC) (5–15%), inlet air temperatures (160–180°C) and feed flow rate (400–600ml/h) to produce powder from beetroot juice concentrate (BJC). The total solids content of BJC was adjusted to 25% (w/w) with the addition of WPC. The obtained powders were analyzed for powder yield, moisture content, hygroscopicity, bulk density, a-value (color), antioxidant activity (AOA), TPC, and betalain content. The results revealed that increasing inlet temperature lowered the bulk density, AOA, TPC and betalain content in terms of 0.590 to 0.482g/ml, 74.75 to 68.85%, 22.23 to 19.36mg GAE/100g and 268.54 to 267.45mg/100g. Depending on the analysis of the results, inlet air temperature, WPC concentration, and the interaction among them had a significant effect on the studied responses (p<0.0001). Feed flow rate on contrary, showed non-significant effect.