The objective of this study was to determine the impact of different slaughter procedures on animal welfare and meat quality. Before slaughter in a dip-lift, one-gondola system, 460 pigs were exposed to an atmosphere containing either 80% or 90% CO 2 for 70 or 100s, and at the longer exposure time with stun-to-stick intervals of either 25–35 or 40–50s. Clinical parameters (reflexes, catecholamines and lactate) showed deficiencies in animal welfare after stunning with 80% CO 2 for 70 and 100s, with an interval of 40–50s, and with 90% CO 2 for 70s. Stunning with 80% CO 2 for 70 or 100s always induced stress, as indicated by higher lactate levels, and reduced meat quality as indicated by low pH 24 values (5.4 in Musculus longissimus and 5.5 in Musculus semimembranosus) and low impedance (Py 24 ) values, especially in combination with the longer stun-to-stick interval (40–50s). Stunning with 80% or 90% CO 2 in a dip-lift system was found to be acceptable for animal welfare (percentage of clinical reflexes) only in combination with the longer exposure time of 100s and the shorter stun-to-stick times of 25–35s. When 90% CO 2 was used, the longer stun-to-stick interval of 40–50s also gave results acceptable with regard to animal welfare. Generally, in comparison to 80% CO 2 stunning meat quality was superior (higher pH 24 and Py 24 values) after stunning with 90% CO 2 .