This study analyzes the relationship between the sexual hormones and personality based in biological Zuckerman and Cloninger models in 105 health males. The age range was 17–53years (mean 26.71 +9.68 SEM). The sexual hormones were luteinizing hormone (LH), follicle- stimulating hormone (FSH), total testosterone (TT), free testosterone (FT) and bioavailable testosterone (BT) and sex hormone binding globulin (SHBG). The personality variables were Novelty Seking scale (TCI-R) and Neuroticism-Anxiety –N-, Anx-, Impulsivity –ImpSS-, Sociability –Sy-, Activity –Act- and Aggressivity-Hostility -Agg-Host- of the ZKPQ-69. The results showed that TT, FT, BT and NS and Sy were related negatively with age. NS and Sy as associated with TT, FT and BT. Combining Sy+ImpSS the correlation improved a little. Subjects with extreme scores in NS and Sy+ImpSS obtained higher and significant mean scores on TT, FT and BT. The relations between sexual hormones and personality decreased after controlling the age.