Introduction: Cellular mechanisms of generation of B memory cells (Bm) to T-independent antigens of type 2 (TI-2) are still unclear. The present study was done to develop an experimental system for investigation of this process.Materials and Methods: Experiments were performed on CBA mice. DNP-Ficoll was used as TI-2 antigen. Total splenocytes or different cell subpopulations were cultured with or without the antigen for 4 days at 37°C, washed and transferred into unimmunized syngeneic mice. One month later mice were immunized by the antigen in vivo. Antibody-forming cells (AFC) were determined by the local hemolysis method. Anti-DNP antibodies were determined by ELISA.Results: The transfer of immune splenocytes resulted in two-fold increase of immune response to DNP as compared with that of recipients of normal splenocytes. Anti-DNP antibodies in recipient sera were not only of IgM, but also of IgG isotypes (of all subclasses). This effect was due mainly to adherent cells, but not to T or B cells; the transfer of primed adherent cells induced the same increase in AFC number and the appearance of the same isotopes of antibodies. These data can be explained by more efficient presentation of DNP-Ficoll by primed adherent cells.Conclusion: The experimental system described can be used for the investigation of Bm cell generation and for study the role of different cell subpopulations in this process.