Journal of Clinical Virology

Items from 1 to 16 out of 16 results

article

Letter to the Editor

W. Lawrence Drew

Journal of Clinical Virology > 2005 > 33 > 1 > 79

article

Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and Pyrosequencing

Martin E. Adelson, Melanie Feola, Jason Trama, Richard C. Tilton, more

Journal of Clinical Virology > 2005 > 33 > 1 > 25-34

Up to 80% of the US adult population has been exposed to herpes simplex virus (HSV) type 1, primarily during childhood. Also, approximately 20% of the US population has contracted genital herpes from HSV-2 infections. Clinical symptoms can present as fever, headache, malaise, myalgia, and cold sores/lesions that cause pain, itching, dysuria, and vaginal or urethral discharge. A recurrence of infection...

article

The emergence of rotavirus genotype G9 in hospitalised children in Slovenia

A. Steyer, M. Poljšak-Prijatelj, D. Barlič-Maganja, T. Bufon, more

Journal of Clinical Virology > 2005 > 33 > 1 > 7-11

Rotavirus G9 genotype was thought to be the fifth most common genotype circulating amongst the population. In previous studies in Slovenia, only G1, G3 and G4 genotypes were detected.To determine G and P genotypes of rotaviruses causing dehydrating gastroenteritis in children hospitalised at the University Medical Centre Ljubljana during the winter season 2001–2002. Some data obtained in previous...

article

Predominance of rotavirus G9 genotype in children hospitalized for rotavirus gastroenteritis in Belgium during 1999–2003

Mustafizur Rahman, Jelle Matthijnssens, Truus Goegebuer, Karolien De Leener, more

Journal of Clinical Virology > 2005 > 33 > 1 > 1-6

Group A rotavirus genotypes G1, G2, G3 and G4 are the main etiological agents of infantile diarrhea. The G9 rotavirus has recently emerged as a fifth important genotype all over the world.To characterize the VP7 gene of group A rotaviruses from gastroenteritis patients admitted to the Gasthuisberg University Hospital, Leuven, Belgium, during 1999–2003.Rotavirus antigen was detected in stool specimens...

article

Editorial Listing

Journal of Clinical Virology > 2005 > 33 > 1 > ii

article

High frequency of parvovirus B19 DNA in bone marrow samples from rheumatic patients

Anders Lundqvist, Adiba Isa, Thomas Tolfvenstam, Göran Kvist, more

Journal of Clinical Virology > 2005 > 33 > 1 > 71-74

Human parvovirus B19 (B19) polymerase chain reaction (PCR) is now a routine analysis and serves as a diagnostic marker as well as a complement or alternative to B19 serology. The clinical significance of a positive B19 DNA finding is however dependent on the type of tissue or body fluid analysed and of the immune status of the patient.To analyse the clinical significance of B19 DNA positivity in bone...

article

Follow-up of 28 HCMV seropositive renal-transplant recipients: comparison of clinical, biological and virological parameters in the groups of treated versus untreated infected patients

Nassim Kamar, Catherine Mengelle, Salah Yahyaoui, Karine Sandres-Sauné, more

Journal of Clinical Virology > 2005 > 33 > 1 > 35-42

A pre-emptive strategy for prevention of Human Cytomegalovirus (HCMV) disease depends on accurate detection of HCMV infection and clinical and/or biological abnormalities.The aim of our study was to evaluate a therapeutic strategy based on the presence of either minimal clinical and/or biological symptoms or high HCMV viral load assessed by quantitative real-time PCR from whole blood as previously...

article

Clinical outcome of long-term lamivudine treated chronic HBV infected and HIV/HBV coinfected patients

Ioannis S. Elefsiniotis, V. Paparizos, S.P. Dourakis, C. Mpotsi, more

Journal of Clinical Virology > 2005 > 33 > 1 > 80-82

article

Molecular epidemiology of HTLV-2 infection among intravenous drug users in Spain

Carlos Toro, Berta Rodés, Sylvina Bassani, Victoria Jiménez, more

Journal of Clinical Virology > 2005 > 33 > 1 > 65-70

HTLV-2 infection is prevalent among intravenous drug users (IDUs), which often are coinfected with HIV-1. Two main subtypes, HTLV-2a and -2b, have been described among European IDUs, with a characteristically geographic distribution: IIa in northern Europe and IIb in the south. Although frequent traveling within Europe might have favoured a wide dissemination of diverse HTLV-2 variants, HTLV-2b was...

article

Part III. Notes from the 20th Annual Clinical Virology Symposium, April 25–28, 2004, Clearwater Beach, FL, USA

Ralf D. Hess, Barbara C. Gärtner, Patricia E. Garrett

Journal of Clinical Virology > 2005 > 33 > 1 > 83-87

article

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods

W.C. Yam, K.H. Chan, K.H. Chow, L.L.M. Poon, more

Journal of Clinical Virology > 2005 > 33 > 1 > 19-24

The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease.To evaluate an automatic nucleic acid extraction system...

article

Comparative evaluation of the Cobas Amplicor HIV-1 Monitor™ Ultrasensitive Test, the new Cobas AmpliPrep/Cobas Amplicor HIV-1 Monitor™ Ultrasensitive Test and the Versant HIV RNA 3.0 assays for quantitation of HIV-1 RNA in plasma samples

Annemarie Berger, Lina Scherzed, Martin Stürmer, Wolfgang Preiser, more

Journal of Clinical Virology > 2005 > 33 > 1 > 43-51

There are several commercially available assays for the quantitation of HIV RNA. A new automated specimen preparation system, the Cobas AmpliPrep™, was developed to automate this last part of the PCR.We compared the results obtained by the Roche Cobas Amplicor HIV-1 Monitor Ultrasensitive Test (MCA, manual sample preparation) with those by the Versant HIV-1 RNA 3.0 assay (bDNA). Secondly we compared...

article

Mitochondrial DNA depletion in adipose tissue of HIV-infected patients with peripheral lipoatrophy

Marc Buffet, Michaël Schwarzinger, Bahia Amellal, Karine Gourlain, more

Journal of Clinical Virology > 2005 > 33 > 1 > 60-64

NRTI-induced host toxicity is proposed to involve cellular mitochondrial DNA (mtDNA) depletion. Determinants of cellular mtDNA copy number from HIV-infected patients receiving HAART and HIV-seronegative controls were investigated from subcutaneous fat samples, and relation with antiretroviral regimen was studied.HIV-infected patients receiving HAART (n=50), HIV-infected patients not currently under...

article

Molecular characterization of Kaposi's sarcoma associated herpesvirus (KSHV) from patients with AIDS-associated Kaposi's sarcoma in São Paulo, Brazil

Maria C. Nascimento, Natalie Wilder, Claudio S. Pannuti, Helen A. Weiss, more

Journal of Clinical Virology > 2005 > 33 > 1 > 52-59

Kaposi's sarcoma (KS) is caused by Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8), the eighth Herpesvirus found to infect humans. The molecular epidemiology of KSHV is related closely to ethnicity and geographical location of studied populations. There is little epidemiological and molecular information about KSHV strains circulating in Brazil.To characterize KSHV strains isolated from AIDS...

article

Intermittent viremia during first-line, protease inhibitors-containing therapy: significance and relationship with drug resistance

Bernard Masquelier, Edwige Pereira, Gilles Peytavin, Diane Descamps, more

Journal of Clinical Virology > 2005 > 33 > 1 > 75-78

In HIV-infected patients on first-line antiretroviral therapy, the significance of intermittent viremia and their relationship with drug resistance remain unclear.To study the virological characteristics of intermittent viremia (IV) and the association between IV and later virological failure (VF) in patients on a first-line, PI-containing therapy.Antiretroviral-naïve patients were enrolled in the...

article

Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

Jincun Zhao, Wei Wang, Guang-Fa Wang, Yonghua Li, more

Journal of Clinical Virology > 2005 > 33 > 1 > 12-18

Severe acute respiratory syndrome (SARS) is caused by infection with SARS-associated coronavirus (CoV). Amino acid residues 450–650 of the spike (S) glycoprotein of SARS-CoV (S450-650) contains dominant epitopes for anti-viral antibodies (Abs) in patient sera.To develop and evaluate an ELISA system for detection of anti-S Abs in patient sera.Express recombinant S450-650 in E. Coli and evaluate the...

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33
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2005

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Keywords

HIV (2)
PCR (2)
REAL-TIME PCR (2)
ROTAVIRUS (2)
ADHERENCE (1)
ADIPOSE TISSUE (1)
AIDS (1)
ANTIBODIES (1)
ANTIRETROVIRAL THERAPY (1)
ARTHRITIS (1)
AUTOMATION (1)
BONE MARROW (1)
BRAZIL (1)
CLINICAL AND BIOLOGICAL ABNORMALITIES (1)
DDNA (1)
DIAGNOSIS (1)
ELISA (1)
G-SEROTYPE (1)
G9 (1)
GENOTYPE (1)
HCMV SEROPOSITIVE RENAL-TRANSPLANT RECIPIENTS (1)
HERPES SIMPLEX VIRUS (1)
HHV-8 (1)
HIV DRUG RESISTANCE (1)
HTLV-2 (1)
IDUS (1)
INTERMITTENT VIREMIA (1)
KAPOSI'S SARCOMA (1)
KSHV (1)
MITOCHONDRIAL DNA (1)
MOLECULAR EPIDEMIOLOGY (1)
OUTCOME (1)
PARVOVIRUS B19 (1)
PERIPHERAL LIPOATROPHY (1)
PERSISTENCE (1)
PHYLOGENY (1)
PRE-EMPTIVE TREATMENT (1)
PROTEASE INHIBITORS (1)
PYROSEQUENCING (1)
QUANTITATION (1)
QUANTITATIVE REAL-TIME PCR (1)
RAPID DIAGNOSIS (1)
RT-PCR (1)
SARS CORONAVIRUS (1)
SARS-CORONAVIRUS (1)
SPIKE GLYCOPROTEIN (1)
SUBTYPE (1)
WORKFLOW (1)
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