A hollow fiber liquid phase microextraction (HF-LPME) and gas chromatographic-electron capture detection (GC-ECD) method for the determination of six fungicides (chlorothalonil, hexaconazole, penconazole, procymidone, tetraconazole, and vinclozolin) in 3ml of water was described. The method used 3μl of toluene as extraction solvent, 20min extraction time with pH 4, stirring at 870rpm, and no salt addition. The enrichment factors of this method were from 135 to 213. Limits of detection were in the range of 0.004–0.025μg/l. The relative standard deviations (RSDs) at 0.1 and 5μg/l of spiking levels were in the range 3–8%. Recoveries of six fungicides from farm water at a spiking level of 0.5μg/l were between 90.7 and 97.6%. The method compared favorably with the traditional method in terms of the sample size, analysis time, and cost.