Sera from yellow croaker (Pseudosciaena crocea), which were collected from the cage-farming area of Xiangshan Bay in China during recovery process after the super typhoon “Krosa,” were studied by Ultra Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry (UPLC/Q-TOF-MS) using electron spraying ionization with positive and negative modes, in an effort to find potential metabolic biomarkers and reveal their physiological changes. After the UPLC/Q-TOF-MS analysis, Principal Components Analysis (PCA) and Orthogonal Projection to Latent Structures Discriminant Analysis (O-PLS-DA) were achieved using SIMCA-P for marker selection and identification. According to the data, the main biomarkers were identified as phosphatidylcholine and lysophosphatidylcholine. The others were characterized as cortol and taurocholate. In the whole half a month period of the study, lysophosphatidylcholine, phosphatidylcholine with higher unsaturated acyl chains (n > 9) and taurocholate showed time-dependent increasing trends, whereas phosphatidylcholine with lower unsaturated acyl chains (n < 8) and cortol showed decreasing trends. These changes in metabolic biomarker are attributed to corticoid regulation of lipid in Pseudosciaena crocea during recovery process after typhoon