Data is presented showing that molecules with identical or nearly identical fluorescence spectra (isochromatic) can be distinguished based on their fluorescence polarization in surfactant solutions. In this report, the steady-state fluorescence polarizations of a series of xanthene dyes (fluorescein (FLR), rhodamine 123 hydrate (R123H), rhodamine B, rhodamine B isocyanate and rhodamine 6G (R6G)) were measured in surfactant solutions consisting of cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Triton X100 (TX-100). Fluorophores exhibited different steady-state polarizations based on their affinities for the micellar environment.